SENIOR TECHNICIAN - NATIONAL FACILITY FOR GENOME ENGINEERING

APPLICATION CLOSING DATE.
July 1st, The Human Technopole (HT) is a new interdisciplinary research institute in Milan, Italy, created and supported by the Italian government whose aim is to develop innovative strategies to promote human health through a multidisciplinary and integrated approach, combining genomics, computational and structural biology, neuroscience, and data and decisions sciences.
HT has the mission of conducting cutting-edge research but also to translate innovation into methods and tools available to the entire National scientific community.
To this end, HT is opening National Facilities that will provide state-of-the-art research infrastructure for Structural Biology, Light Imaging, Genomics, Genome Engineering, and Data Handling and Analysis.
Overview – National Facility for Genome Engineering and Disease Modelling The core mission of the National Facility for Genome Engineering and Disease Modelling is to enable patient-specific disease modelling at scale by translating, benchmarking and standardising laboratory protocols into automated workflows, integrating state-of-the-art robotic and bioengineering platforms through a highly multidisciplinary approach.
Operating in close collaboration with the Genomics and Advanced Imaging National Facilities, as well as the Flow Cytometry and Electrophysiology support units, GEDI develops transformative technologies to achieve the Human Technopole vision of high-definition biomedicine.
This involves utilizing increasingly sophisticated organoid systems at single-cell multiomic resolution, with a strong emphasis densely phenotyped clinical cohorts.
The successful candidate will report to the Head of the Facility and will have the opportunity to work in a multidisciplinary environment to develop and support the execution of experimental activities in the following areas.
Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs), followed by characterization and banking of the generated iPSCs Key tasks and responsibilities.
Carry out, in synergy with the facility team, the experimental activities envisaged for the provision of Facility services in the field of stem cell and organoid cultures.
Develop and optimize cell culture protocols for the differentiation of stem cells into organoids, combining state-of-the-art methods of molecular biology, cell biology and organoid technologies.
Develop innovative solutions to adapt conventional cell culture routines to miniaturized, automation-friendly protocols.
Generating/importing new tools and protocols.
Supporting the head of the Facility in setting up core resources, participating in key aspects of general facility management and organization (e.
., managing inventories and stocks of samples, reagents, equipment and materials) Mandatory requirements.
A PhD in molecular biology, genetics, biochemistry, or in a related field of the life sciences is required.
At least 5 years of relevant work experience.
Advanced cell culture experience with human pluripotent stem cell lines and organoids.
Experience and skills in performing repetitive and specialized laboratory experimental procedures.
Fluency in English – HT is an international research institute.
Preferred requirements.
Experience in molecular biology (DNA and RNA manipulation, cloning, sequencing, RT-PCR, single cell manipulation, FACS).
Experience in tissue processing and histology applied to mammalian tissues and organoids.
Familiarity with Protein Biochemistry methods (immunoblotting and immunoprecipitation techniques, preferably also recombinant protein expression, purification and in vitro reactions).
Experience with iPS cell reprogramming from somatic cells (e.
.
PBMCs).
Experience in brain modelling using different organoid protocols.
Experience in xenotransplantation techniques.
Organizational and social skills.
Ability to manage competing priorities in a fast-paced environment and to work independently.
Ability to present project and results to the team as well as a scientific literate audience.
Strong interpersonal skills demonstrated by the ability to lead and gain the assistance and cooperation of others in a team endeavour.
Ability to build trust through operating with transparency and creating an open and positive environment.
Ability to work in a multi-cultural, multi-ethnic environment with sensitivity and respect for diversity.
Application Instructions Please apply online sending.
A full CV.
A motivation letter.
Referee contacts WHY HUMAN TECHNOPOLE? HT seeks for scientific excellence, we recruit the best scientific talents through international, open calls and meritocratic selection procedures.
Our working environment is international, friendly and inclusive.
Our scientists work together across disciplines on research topics of biomedical relevance, leveraging synergies between their diverse skillsets and methodological approaches.
We believe that highly diverse teams yield the best and most innovative results.
We engage in outward-facing scientific activities aimed at benefiting the national and international research community.
Training is also at the heart of our activity, with initiatives and opportunities for our staff, including scientific courses, conferences and workshops.
Main benefits Welfare plans.
Canteen service.
Work-life balance provisions.
Italian language training for foreigners.
Maternity leave up to 1 year and other support for new parents.
Possibility of flexible working hours Support for relocation.
Special consideration will be given to candidates who are part of the protected categories list, according to L.
.
Number of positions offered.
1 Contract offered.
CCNL Chimico Farmaceutico, 3 years Fixed-term – Employee level Salary range.
up to (RAL) depending on the seniority.
Tax benefits where applicable.
Tax benefits where applicable.
The position is based in Milan “The Foundation reserves the right, at its sole discretion, to extend, suspend, modify, revoke, or cancel this job posting without giving rise to any rights or claims whatsoever in favor of the candidates; the Foundation reserves, however, the right not to proceed with the awarding of the above-described assignment due to the effect of supervening regulatory provisions and/or obstructive circumstances”.
Tagged as.
Engineering