LABORATORY TECHNICIAN CELL BIOLOGY

The research group of Precision Medicine led by Prof. Salvatore Piscuoglio ( ) will be based al the Humanitas University, Milan, Italy, from July 2024 is seeking one highly motivated Laboratory Technicians in Cell Biology (100%) to join our team of bioinformaticians, molecular biologists, pathologists, and clinical fellows. The candidates will develop, optimize and apply cutting-edge cellular/ molecular methods to generate 3D in vitro and ex vivo tumor models that adequately recapitulate the tumor architecture and mimic the cell-to-cell interactions within the tissue microenvironment. The specific tasks will include the generation and maintenance of patient-derived tumor organoids, patient-derived tumor fragments, fibroblasts, endothelial cells, Peripheral Blood Mononuclear Cells (PBMCs), and Tumor Infiltrating Lymphocytes (TILs). Main responsibilities include processing patient-derived samples according to pre-established guidelines (e.g., tissue dissociation, isolation of PBMCs/ TILs, endothelial cells, sample freezing and storage, nucleic acids and protein extraction, and patient-derived tumor organoid/ fragment generation and maintenance) generation and maintenance of cell cultures and execution of experiments on cell cultures updating the database of patient samples and reagents documenting the experiments providing general support for lab maintenance within the scope of this project Profile "Laboratory Technician" degree or a Bachelor/Master degree in molecular biology, biotechnology or a related discipline excellent technical expertise in molecular/ cell biology techniques hands-on experience handling and processing patient samples hands-on experience with cell culture and flow cytometry techniques is a must experience in primary cell culture and 3D cultures is highly desirable technical expertise in organoid generation and maintenance is a plus once trained, the ability to work independently organized and detail-oriented; ability to maintain accurate documentation of experiments and sample information collaborative; ability to work effectively in a team. good organizational skills and ability to multitask fluency in English is a must Offer These positions offer exciting opportunities for highly motivated individuals to contribute to advancing personalized medicine in cancer. We offer the opportunity to expand your portfolio with cutting-edge skills in 3D tumor model generation. We offer a stimulating and international research environment with a strong and dynamic network of international collaborators. Humanitas University offers dedicated training programs to broaden your expertise and enhance your skill set. All candidate data collected from the application shall be processed in accordance with applicable law: Dlgs 198/2006 e dei Dlgs 215/2003 e 216/2003; privacy ex artt. 13 e 14 del Reg. UE 2016/679.