INDENTIFICATION OF NOVEL TARGETS TO DEVELOP INNOVATIVE THERAPEUTIC APPROACHES FOR BRITTLE BONE [...]

Organisation/Company: Università di Pavia Research Field: Biological sciences Researcher Profile: Recognised Researcher (R2), Leading Researcher (R4), First Stage Researcher (R1), Established Researcher (R3) Country: Italy Application Deadline: 27 Nov 2024 - 23:59 (UTC) Type of Contract: To be defined Job Status: Not Applicable Is the job funded through the EU Research Framework Programme? Not funded by a EU programme Is the Job related to staff position within a Research Infrastructure? No Offer Description The project will focus on studying the role of ER-phagy in modulating phenotypic severity in the disease Osteogenesis Imperfecta (OI). OI, the most common among hereditary bone diseases, is caused by dominant mutations in the COL1A1 and COL1A2 genes which encode the α chains of collagen I. These mutations cause partial retention of the mutated protein in the endoplasmic reticulum (ER). A type of autophagy, defined as ER-phagy, appears to be involved in reducing the accumulation of poorly folded proteins in the ER, including collagen. The aim of the project will be to analyze the role and in vivo regulation of ER-phagy in the clearance of misfolded collagen I molecules in OI. Animal models of OI will be used to study and identify key regulators of ER phage processing and its contribution to cellular responses associated with intracellular accumulation of collagen I. Main Requirements Degree (with a course duration of not less than 4 years, as referred to in the pre-existing academic regulations pursuant to D.M. n. 509/1999) or post-graduate degrees (pursuant to D.M. n. 509/1999 and to n. 270/2004) awarded by Italian or foreign Universities recognized as equivalent. Scientific or professional curriculum suitable for conducting the research activity. If available, any Ph.D. title (Art 2- par. 1). Further Specific Assessable Qualifications Proven experience of cell cultures of eukaryotic cells Proven experience in the use of biochemical and molecular techniques (including but not limited to western blotting, real-time PCR) Proven experience in the use of histological techniques on tissues (tissue preparation, embedding, sections and stains or immunohistochemistry) Proven experience in the use of fluorescence microscopy (confocal and super-resolution) Proven experience in the use of animal models Excellent knowledge of the English language International publications on topics relating to the characterization of animal models of skeletal diseases Selection Process The application for admission to the selection procedure (dated and signed), the documents and publications considered useful for the evaluation must be submitted electronically, by visiting the following web page: by choosing the “Assegni di ricerca” item and selecting the Competition Notice you have chosen. In order to register with the system, you must have an email address. Following the guidelines published in the procedure, the applicant must enter all the information required for the application, enclosing all documents in electronic .PDF format. The selection of candidates is carried out on the basis of qualifications, project and interview, supplemented by a test of knowledge of the foreign language (see art. 6 - paragraph 1). #J-18808-Ljbffr